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A Single‐Stranded Junction Modulates Nanosecond Motional Ordering of the Substrate Recognition Duplex of a Group I Ribozyme
Author(s) -
Nguyen Phuong,
Shi Xuesong,
Sigurdsson Snorri Th.,
Herschlag Daniel,
Qin Peter Z.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300376
Subject(s) - ribozyme , nanosecond , duplex (building) , substrate (aquarium) , group (periodic table) , hairpin ribozyme , biophysics , chemistry , nanotechnology , rna , materials science , physics , biology , dna , biochemistry , optics , organic chemistry , ecology , laser , gene
Rigid spinning: Site‐directed spin‐labeling studies using a rigid nitroxide spin label ( Ç ) reveal that both length and sequence of a single‐stranded junction (J1/2) modulate nanosecond motional ordering of the substrate‐recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high‐molecular‐weight RNA complexes.