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DNA Binding and Bending by Sac7d is Stepwise
Author(s) -
Spiriti Justin,
van der Vaart Arjan
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300264
Subject(s) - sulfolobus acidocaldarius , dna , binding energy , bending , chemistry , biophysics , thermodynamics , physics , biology , biochemistry , atomic physics , gene , archaea
Which came first? Using thermodynamic cycles constructed from experimental measures of the binding free energy and free energy simulations, we have shown that binding of Sulfolobus acidocaldarius protein Sac7d to DNA occurs before DNA bending, thus indicating that a conformational selection mechanism is unlikely to be operative in Sac7d binding.