A Computational Study of the Effects of  13 C– 13 C Scalar Couplings on  13 C CEST NMR Spectra: Towards Studies on a Uniformly  13 C‐Labeled Protein | Zendy

Vallurupalli Pramodh | Zendy; Bouvignies Guillaume | Zendy; Kay Lewis E. | Zendy

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A Computational Study of the Effects of 13 C– 13 C Scalar Couplings on 13 C CEST NMR Spectra: Towards Studies on a Uniformly 13 C‐Labeled Protein

Author(s) -

Vallurupalli Pramodh,

Bouvignies Guillaume,

Kay Lewis E.

Publication year - 2013

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201300230

Subject(s) - spectral line , physics , carbon 13 , nmr spectra database , spin (aerodynamics) , nuclear physics , nuclear magnetic resonance , atomic physics , chemistry , quantum mechanics , thermodynamics

Read the label: The NMR CEST experiment can be used to reconstruct spectra of sparsely populated, transiently formed protein conformers so long as they exchange with a highly populated ground state with rates of 20–300 s −1 . Here we establish that accurate 13 C chemical shifts of side‐chain carbon nuclei can be obtained from uniformly 13 C‐labeled samples, without interference from the coupled 13 C spin network.

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