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In Vivo Evidence for a Prodrug Activation Mechanism during Colibactin Maturation
Author(s) -
Bian Xiaoying,
Fu Jun,
Plaza Alberto,
Herrmann Jennifer,
Pistorius Dominik,
Stewart A. Francis,
Zhang Youming,
Müller Rolf
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300208
Subject(s) - prodrug , in vivo , escherichia coli , mechanism (biology) , chemistry , asparagine , biochemistry , gene cluster , cleavage (geology) , biology , gene , genetics , enzyme , philosophy , epistemology , paleontology , fracture (geology)
Releasing the cytopath: We have identified an N ‐myristoyl‐ D ‐asparagine ( 1 ) as the free N‐terminal prodrug scaffold in cytopathogenic Escherichia coli strains expressing the colibactin gene cluster. Colibactin is released in vivo upon cleavage of precolibactin. We provide for the first time in vivo evidence of the prodrug‐like release mechanism of colibactin.