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Development of a Novel FRET Probe for the Real‐Time Determination of Ceramidase Activity
Author(s) -
Bhabak Krishna P.,
Hauser Anett,
Redmer Susanne,
Banhart Sebastian,
Heuer Dagmar,
Arenz Christoph
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300207
Subject(s) - förster resonance energy transfer , chemistry , sphingolipid , computational biology , nanotechnology , biophysics , biochemistry , biology , materials science , fluorescence , physics , quantum mechanics
Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real‐time.