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Structures of KOD and 9°N DNA Polymerases Complexed with Primer Template Duplex
Author(s) -
Bergen Konrad,
Betz Karin,
Welte Wolfram,
Diederichs Kay,
Marx Andreas
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300175
Subject(s) - duplex (building) , primer (cosmetics) , dna polymerase , dna , enzyme , nucleotide , polymerase , primase , biochemistry , chemistry , biology , computational biology , microbiology and biotechnology , polymerase chain reaction , reverse transcriptase , gene , organic chemistry
Replicate it: Structures of KOD and 9°N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates.