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Fluorescent Probes for Live Cell Imaging of Endogenous Guanine Nitration
Author(s) -
Saito Yohei,
Ito Chiaki,
Fujii Shigemoto,
Sawa Tomohiro,
Akaike Takaaki,
Arimoto Hirokazu
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300129
Subject(s) - fluorescence , cysteine , computer science , chemistry , endogeny , mechanism (biology) , biochemistry , computational biology , nanotechnology , microbiology and biotechnology , combinatorial chemistry , biology , philosophy , materials science , physics , enzyme , epistemology , quantum mechanics
Seeing is believing: S‐guanylation is a novel key mechanism by which signal transduction under oxidative stress is regulated. A chemical probe whose fluorescent intensity increases after the reaction with proteinous cysteine (S‐guanylation) is described. The use of this probe revealed that S‐guanylation products localized in lysosomes.