Nucleobase Protection Strategy for Gene Cloning and Expression | Zendy

Kielkowski Pavel | Zendy; Brock Nelson L. | Zendy; Dickschat Jeroen S. | Zendy; Hocek Michal | Zendy

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Nucleobase Protection Strategy for Gene Cloning and Expression

Author(s) -

Kielkowski Pavel,

Brock Nelson L.,

Dickschat Jeroen S.,

Hocek Michal

Publication year - 2013

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201300127

Subject(s) - cloning (programming) , plasmid , restriction enzyme , gene , nucleobase , molecular cloning , cleavage (geology) , microbiology and biotechnology , protecting group , chemistry , dna , biology , synthetic biology , genetics , computational biology , gene expression , paleontology , alkyl , organic chemistry , fracture (geology) , computer science , programming language

Protecting group chemistry meets molecular biology: Chemically modified dATP carrying a bulky triethylsilylethynyl group was used in a PCR‐based synthesis of a gene internally protected against cleavage by restriction endonucleases. The unmodified flanking regions were cleaved for cloning into a plasmid which was replicated by E. coli , and used for protein production.

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