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A Click‐and‐Release Pyrrolysine Analogue
Author(s) -
Lee Marianne M.,
Fekner Tomasz,
Tang TszHo,
Wang Lin,
Chan Aurora HoYin,
Hsu PangHung,
Au Shan W.,
Chan Michael K.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300124
Subject(s) - click chemistry , computer science , sumo protein , combinatorial chemistry , chemistry , computational biology , biochemistry , biology , ubiquitin , gene
What's the catch? A pyrrolysine analogue bearing a terminal alkyne and an ester functionality can be incorporated into recombinant proteins and render them amenable to capture by the click reaction and subsequent release through ester hydrolysis. The utility of this pyrrolysine‐inspired technology is demonstrated for the identification of SUMOylation sites.