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Making Ends Meet: Chemically Mediated Circularization of Recombinant Proteins
Author(s) -
Cowper Ben,
Craik David J.,
Macmillan Derek
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300105
Subject(s) - library science , art history , sociology , history , computer science
A selective N→S acyl transfer reaction facilitates semi‐synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N‐terminal cysteine and a thiol‐labile C‐terminal Gly‐Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.