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A Label‐Free, Quantitative Assay of Amyloid Fibril Growth Based on Intrinsic Fluorescence
Author(s) -
Pinotsi Dorothea,
Buell Alexander K.,
Dobson Christopher M.,
Kaminski Schierle Gabriele S.,
Kaminski Clemens F.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300103
Subject(s) - amyloid fibril , fluorescence , chemistry , biophysics , fibril , amyloid (mycology) , amyloid β , biochemistry , biology , medicine , pathology , inorganic chemistry , physics , disease , quantum mechanics
Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α‐synuclein seeds with α‐synuclein monomeric protein (blue curve) but not when α‐synuclein seeds are incubated with β‐synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.