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Widening the pH Activity Profile of a Fungal Laccase by Directed Evolution
Author(s) -
TorresSalas Pamela,
Mate Diana M.,
Ghazi Iraj,
Plou Francisco J.,
Ballesteros Antonio O.,
Alcalde Miguel
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300102
Subject(s) - directed evolution , laccase , selection (genetic algorithm) , chemistry , computer science , mutant , world wide web , biochemistry , enzyme , gene , artificial intelligence
Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.