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Highly Efficient Förster Resonance Energy Transfer in a Fast, Serum‐Compatible Immunoassay
Author(s) -
Kreisig Thomas,
Hoffmann Ralf,
Zuchner Thole
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300073
Subject(s) - förster resonance energy transfer , immunoassay , phosphorescence , homogeneous , acceptor , linear range , energy transfer , detection limit , chemistry , analytical chemistry (journal) , materials science , nanotechnology , chromatography , fluorescence , optics , physics , chemical physics , antibody , immunology , biology , condensed matter physics , thermodynamics
Highly efficient FRET leads to important enhancements for homogeneous immunoassays. By using the novel phosphorescent dye EuLH and BHQ‐10 as a donor–acceptor pair, the FRET efficiency increases to >99.5 %, leading to significantly improved signal‐to‐background ratio, precision and linear range. The phosphorescence detection enabled full compatibility to serum samples for this fast‐responding immunoassay.