Premium
A Trans ‐Acting Ketoreductase in Biosynthesis of a Symmetric Polyketide Dimer SIA7248
Author(s) -
Zou Yi,
Yin Haixing,
Kong Dekun,
Deng Zixin,
Lin Shuangjun
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300068
Subject(s) - polyketide , biosynthesis , stereochemistry , gene cluster , polyketide synthase , dimer , stereospecificity , chemistry , strain (injury) , trans acting , streptomyces , biochemistry , gene , biology , bacteria , genetics , organic chemistry , catalysis , mutant , anatomy
New assembly line, new compound: SIA7248, a new symmetric macrolide, was isolated from a marine‐derived Streptomyces strain. Bioinformatic analyses of the identified biosynthetic gene cluster ( sia ) for SIA7248 suggested a polyketide biosynthesis utilizing an iteratively trans ‐acting ketoreductase (KR). We characterized SiaM as a trans ‐KR to catalyse reductions of various β‐ketoacyl‐thioesters with D ‐stereospecificity.