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Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays
Author(s) -
Serna Sonia,
Hokke Cornelis H.,
Weissenborn Martin,
Flitsch Sabine,
MartinLomas Manuel,
Reichardt NielsChristian
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300051
Subject(s) - glycan , glycosyltransferase , tritium , chemistry , dna microarray , profiling (computer programming) , computational biology , glycoside , microarray , combinatorial chemistry , biochemistry , stereochemistry , biology , gene , computer science , glycoprotein , gene expression , programming language , physics , nuclear physics
High‐throughput microarray technology has been combined with ultrasensitive and high‐resolution tritium autoradiography to create a new platform for the quantitative detection of glycosyltransferase activity on glycan arrays. In addition, we show full compatibility with the use of fluorescently labeled lectins to help with the stereochemical assignment of newly formed glycoside linkages.