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Photobleaching Lifetimes of Cyanine Fluorophores Used for Single‐Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems
Author(s) -
Cooper David,
Uhm Heui,
Tauzin Lawrence J.,
Poddar Nitesh,
Landes Christy F.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300030
Subject(s) - photoprotection , photobleaching , cyanine , förster resonance energy transfer , computer science , photobiology , chemistry , energy transfer , single molecule fret , nanotechnology , biophysics , photochemistry , fluorescence , materials science , physics , biology , chemical physics , biochemistry , optics , photosynthesis
Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single‐molecule environment. We found that the glucose/glucose oxygen‐scavenging solution in combination with redox‐based photostabilization solutions yielded the longest average photobleaching lifetimes.