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Selective Fluorogenic Chemosensors for Distinct Classes of Nucleases
Author(s) -
Jung JongWha,
Edwards Sarah K.,
Kool Eric T.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300001
Subject(s) - nuclease , dna , computational biology , computer science , thymine , chemistry , combinatorial chemistry , nanotechnology , biochemistry , biology , materials science
Nuclease sensor trio: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3′‐exonucleases, 5′‐exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.

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