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Back Cover: Single‐Step Reconstitution of Apo‐Hemoproteins at the Disruption Stage of Escherichia coli Cells (ChemBioChem 14/2012)
Author(s) -
Kawakami Norifumi,
Shoji Osami,
Watanabe Yoshihito
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201290061
Subject(s) - heme , escherichia coli , hemeprotein , chemistry , nitric oxide , cytochrome , ruthenium , cover (algebra) , biochemistry , stereochemistry , organic chemistry , catalysis , enzyme , gene , mechanical engineering , engineering
The back cover picture shows how reconstitution of apo‐hemoproteins with synthetic metal complexes is possible at the disruption stage of Escherichia coli cells. On p. 2045 ff. , Y. Watanabe et al. report that a simple addition of manganese and ruthenium porphyrins to E. coli cells immediately prior to homogenization yields reconstituted cytochrome P450BM3 and the heme nitric oxide/oxygen binding domain )