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Cover Picture: Achieving Regio‐ and Enantioselectivity of P450‐Catalyzed Oxidative CH Activation of Small Functionalized Molecules by Structure‐Guided Directed Evolution (ChemBioChem 10/2012)
Author(s) -
Agudo Rubén,
Roiban GheorgheDoru,
Reetz Manfred T.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201290038
Subject(s) - directed evolution , saturated mutagenesis , chemistry , monooxygenase , stereoselectivity , enantioselective synthesis , biocatalysis , stereochemistry , catalysis , mutagenesis , oxidative phosphorylation , combinatorial chemistry , mutant , enzyme , organic chemistry , reaction mechanism , cytochrome p450 , biochemistry , gene
The cover picture shows that regio‐ and enantioselective oxidative CH‐activation of a simple compound such as cyclohex‐1‐ene carboxylic acid methyl ester becomes possible by directed evolution of the monooxygenase P450‐BM3. On p. 1465 ff. , M. T. Reetz et al. describe how, by using structure‐guided iterative saturation mutagenesis at the enzyme's binding pocket, R ‐ and S ‐selective mutants were evolved with formation of the respective chiral alcohols, thereby setting the stage for subsequent stereoselective transformations employing synthetic regents and catalysts. Related substrates react similarly with the same mutants.