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Structure and Biosynthesis of Fimsbactins A–F, Siderophores from Acinetobacter baumannii and Acinetobacter baylyi
Author(s) -
Proschak Anna,
Lubuta Patrice,
Grün Peter,
Löhr Frank,
Wilharm Gottfried,
De Berardinis Veronique,
Bode Helge B.
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200764
Subject(s) - siderophore , acinetobacter baumannii , mutant , acinetobacter , biosynthesis , gene , genome , gene cluster , biochemistry , chemistry , biology , microbiology and biotechnology , bacteria , genetics , antibiotics , pseudomonas aeruginosa
Novel chatechol/hydroxamate siderophores (named “fimsbactins”) were identified in Acinetobacter baumannii ATCC 17978 and Acinetobacter baylyi ADP1. The major compound, fimsbactin A, was isolated from low‐iron cultures of A. baylyi ADP1, and its chemical structure was elucidated by mass spectrometry, and detailed 1 H, 13 C and 15 N NMR spectroscopy. From inverse feeding experiments following HPLC‐MS analysis, the structures of five additional derivatives were elucidated. The gene cluster encoding the fimsbactin synthetase ( fbs ) was identified in both genomes, and mutants in fbs genes in A. baylyi were analyzed, thus allowing prediction of the fimsbactin biosynthesis pathway.