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Selective Fluorescence Detection of Small‐Molecule‐Binding Proteins by Using a Dual Photoaffinity Labeling System
Author(s) -
Sakurai Kaori,
Yamada Rika,
Okada Ayumi,
Tawa Masaki,
Ozawa Shimpei,
Inoue Maia
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200758
Subject(s) - photoaffinity labeling , fluorescence , chemistry , small molecule , biophysics , combinatorial chemistry , biochemistry , binding site , biology , physics , quantum mechanics
Photo opportunity: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co‐reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.
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