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Tetramolecular Fluorescence Complementation for Detection of Specific RNAs in Vitro
Author(s) -
Kellermann Stefanie Julia,
Rath Anna Katharina,
Rentmeister Andrea
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200734
Subject(s) - rna , complementation , computational biology , in vitro , computer science , lysis , sequence (biology) , biology , chemistry , microbiology and biotechnology , gene , biochemistry , phenotype
Short fuses: RNA can be detected sequence specifically by using two RNA binding proteins fused to short strands derived from GFP and an additional large GFP fragment. The tetramolecular system has a high signal‐to‐noise ratio, discriminates between closely related RNA, and works in RNA preparations as well as E. coli cell lysate.