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A Bioorthogonal Chemoenzymatic Strategy for Defined Protein Dendrimer Assembly
Author(s) -
Gupta Kanchan,
Singh Shikha,
Gupta Kallol,
Khan Naeem,
Sehgal Devinder,
Haridas V.,
Roy Rajendra P.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200559
Subject(s) - bioorthogonal chemistry , sortase , dendrimer , click chemistry , chemistry , sortase a , covalent bond , scaffold , bioconjugation , azide , cycloaddition , combinatorial chemistry , conjugated system , scaffold protein , biochemistry , organic chemistry , bacterial protein , computer science , catalysis , polymer , database , gene , signal transduction
Protein dendrimers made easy: We present a chemoenzymatic strategy for the synthesis of well‐defined protein dendrimers in which proteins orthogonally labelled by sortase‐mediated ligation are conjugated to a dendritic scaffold by azide–alkyne cycloaddition. This “sortase‐click” strategy uses readily accessible His 6 ‐tagged proteins and can serve as a powerful method for the covalent assembly of multivalent proteins.