A Bioorthogonal Chemoenzymatic Strategy for Defined Protein Dendrimer Assembly | Zendy

Gupta Kanchan | Zendy; Singh Shikha | Zendy; Gupta Kallol | Zendy; Khan Naeem | Zendy; Sehgal Devinder | Zendy; Haridas V. | Zendy; Roy Rajendra P. | Zendy

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A Bioorthogonal Chemoenzymatic Strategy for Defined Protein Dendrimer Assembly

Author(s) -

Gupta Kanchan,

Singh Shikha,

Gupta Kallol,

Khan Naeem,

Sehgal Devinder,

Haridas V.,

Roy Rajendra P.

Publication year - 2012

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201200559

Subject(s) - bioorthogonal chemistry , sortase , dendrimer , click chemistry , chemistry , sortase a , covalent bond , scaffold , bioconjugation , azide , cycloaddition , combinatorial chemistry , conjugated system , scaffold protein , biochemistry , organic chemistry , bacterial protein , computer science , catalysis , polymer , database , gene , signal transduction

Protein dendrimers made easy: We present a chemoenzymatic strategy for the synthesis of well‐defined protein dendrimers in which proteins orthogonally labelled by sortase‐mediated ligation are conjugated to a dendritic scaffold by azide–alkyne cycloaddition. This “sortase‐click” strategy uses readily accessible His 6 ‐tagged proteins and can serve as a powerful method for the covalent assembly of multivalent proteins.

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