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Coupling Reaction of Indolepyruvic Acid by StaD and Its Product: Implications for Biosynthesis of Indolocarbazole and Violacein
Author(s) -
Asamizu Shumpei,
Hirano Satoshi,
Onaka Hiroyasu,
Koshino Hiroyuki,
Shiro Yoshitsugu,
Nagano Shingo
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200535
Subject(s) - biosynthesis , coupling (piping) , substrate (aquarium) , chemistry , natural product , stereochemistry , enzyme , combinatorial chemistry , biochemistry , biology , materials science , ecology , metallurgy
Central CC coupling : It has been proposed that the heme‐containing enzyme StaD and its homologues catalyze a coupling reaction that yields an indolepyruvic acid (IPA) imine in indolocarbazole biosynthesis. We show that StaD uses IPA enol as an analogue of natural substrate to produce an IPA dimer, in the first direct evidence of the CC coupling dimerization reaction catalyzed by StaD.