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A Novel Method for the Labelling of Peptides and Proteins through a Bioorthogonal Staudinger Reaction by Using 2‐Cyanoethyl Phosphoramidites
Author(s) -
Uzagare Matthew C.,
Claußnitzer Iris,
Gerrits Michael,
Bannwarth Willi
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200528
Subject(s) - bioorthogonal chemistry , labelling , chemistry , combinatorial chemistry , biochemistry , click chemistry
Chemoselective modification of biomolecules: The reaction between 2‐cyanoethyl phosphoramidites and azides is economical and can be performed in different solvents, including aqueous buffers. The course of the reaction with azido‐modified amino acids, peptides or proteins and different label molecules was followed by 31 P NMR spectroscopy.