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Single‐Step Reconstitution of Apo‐Hemoproteins at the Disruption Stage of Escherichia coli Cells
Author(s) -
Kawakami Norifumi,
Shoji Osami,
Watanabe Yoshihito
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200446
Subject(s) - hemeprotein , porphyrin , chemistry , escherichia coli , heme , biochemistry , combinatorial chemistry , enzyme , gene
Hemoproteins on their metal: We report a novel strategy for the reconstitution of hemoproteins with non‐natural metal complexes; simple addition of manganese and ruthenium porphyrin to E. coli cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins.