Premium
Probing Translation Initiation through Ligand Binding to the 5′ mRNA Coding Region
Author(s) -
Jo JoonJung,
Kim JooHee,
Shin JongShik
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200432
Subject(s) - eukaryotic translation , translation (biology) , messenger rna , computational biology , ribosome , coding region , ribosomal binding site , riboswitch , annotation , biology , genetics , gene , chemistry , computer science , rna , non coding rna
Secondary structure matters : We have constructed artificial intragenic riboswitches to probe ribosome accessibility to the 5′ mRNA coding region at three‐base resolution in Escherichia coli. We show that only mRNA folding stability in the +1 to +15 nt region affects the translation process.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom