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Probing Translation Initiation through Ligand Binding to the 5′ mRNA Coding Region
Author(s) -
Jo JoonJung,
Kim JooHee,
Shin JongShik
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200432
Subject(s) - eukaryotic translation , translation (biology) , messenger rna , computational biology , ribosome , coding region , ribosomal binding site , riboswitch , annotation , biology , genetics , gene , chemistry , computer science , rna , non coding rna
Secondary structure matters : We have constructed artificial intragenic riboswitches to probe ribosome accessibility to the 5′ mRNA coding region at three‐base resolution in Escherichia coli. We show that only mRNA folding stability in the +1 to +15 nt region affects the translation process.