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Advances in Quantitative FRET‐Based Methods for Studying Nucleic Acids
Author(s) -
Preus Søren,
Wilhelmsson L. Marcus
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200400
Subject(s) - förster resonance energy transfer , nucleic acid , nanotechnology , single molecule fret , chemistry , biological system , materials science , physics , biology , fluorescence , biochemistry , quantum mechanics
Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for “on/off” experiments. To use FRET to obtain quantitative distances and three‐dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single‐molecule levels.