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Selective Desulfurization Significantly Expands Sequence Variety of 3′‐Peptidyl–tRNA Mimics Obtained by Native Chemical Ligation
Author(s) -
Geiermann AnnaSkrollan,
Micura Ronald
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200368
Subject(s) - peptidyl transferase , native chemical ligation , chemical ligation , transfer rna , ribosome , chemistry , rna , peptide bond , peptide , ribosomal rna , moiety , stereochemistry , bioconjugation , combinatorial chemistry , biochemistry , chemical synthesis , in vitro , gene
Accessible: Functionally complex 3′‐peptidyl–RNA conjugates can be efficiently desulfurized under free radical reaction conditions as demonstrated here. One‐pot procedures for native chemical ligation (NCL) and desulfurization enable sequences for this class of bioconjugates that are inaccessible by other methods.