Premium
19 F MRI Monitoring of Gene Expression in Living Cells through Cell‐Surface β‐Lactamase Activity
Author(s) -
Matsushita Hisashi,
Mizukami Shin,
Mori Yuki,
Sugihara Fuminori,
Shirakawa Masashiro,
Yoshioka Yoshichika,
Kikuchi Kazuya
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200331
Subject(s) - magnetic resonance imaging , cell , gene expression , in vivo , reporter gene , living cell , gene , cell membrane , genetic enhancement , microbiology and biotechnology , nuclear magnetic resonance , chemistry , biology , biophysics , medicine , biochemistry , genetics , radiology , physics
Magnetic resonance imaging provides important intravital information on deep tissues that cannot be visualized by other methods. Although we had previously developed an off/on switching 19 F MRI probe to monitor reporter enzyme activity on the basis of the paramagnetic relaxation enhancement effect, it was difficult to monitor biological events in living cells because the 19 F MRI probe did not permeate living cell membrane. In this study, we have developed a new 19 F MRI system for monitoring gene expression in living cells by exploiting cell‐surface‐displayed β‐lactamase and the specifically designed 19 F MRI probe. By using this system, cellular gene expression was successfully detected by 19 F MRI without cell fixation. This imaging strategy shows promise for monitoring in vivo gene expression, and therefore it could lead to useful technologies for the diagnosis and therapy of various diseases.