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A Need for Speed: Genetic Encoding of Rapid Cycloaddition Chemistries for Protein Labelling in Living Cells
Author(s) -
Schmidt Moritz J.,
Summerer Daniel
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200321
Subject(s) - labelling , cycloaddition , chemistry , fluorescent labelling , amino acid , fluorescence , biochemistry , catalysis , physics , quantum mechanics
Rapid reactions : Several reactants for strain‐promoted cycloaddition reactions (see picture) have been genetically encoded as the side chains of noncanonical amino acids. This results in decisive improvements for the fluorescent labelling of intracellular proteins such as quantitative turnover, completion of labelling reactions within minutes, fluorogenic effects and even partial orthogonality for multicolour labelling.