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Catalyst‐Free and Site‐Specific One‐Pot Dual‐Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids
Author(s) -
Wu Bo,
Wang Zhiyong,
Huang Ying,
Liu Wenshe R.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200281
Subject(s) - hydroxylamine , azide , amino acid , chemistry , protein engineering , catalysis , combinatorial chemistry , computational biology , biochemistry , biology , organic chemistry , enzyme
The genetic incorporation of one azide‐containing and one keto‐containing noncanonical amino acid into a protein at amber and ochre mutation sites respectively, followed by their orthogonal reactions with hydroxylamine‐containing and cyclooctyne‐containing dyes allows highly efficient one‐pot site‐specific dual labeling of the protein in a catalyst‐free fashion.