Catalyst‐Free and Site‐Specific One‐Pot Dual‐Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids | Zendy

Wu Bo | Zendy; Wang Zhiyong | Zendy; Huang Ying | Zendy; Liu Wenshe R. | Zendy

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Catalyst‐Free and Site‐Specific One‐Pot Dual‐Labeling of a Protein Directed by Two Genetically Incorporated Noncanonical Amino Acids

Author(s) -

Wu Bo,

Wang Zhiyong,

Huang Ying,

Liu Wenshe R.

Publication year - 2012

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201200281

Subject(s) - hydroxylamine , azide , amino acid , chemistry , protein engineering , catalysis , combinatorial chemistry , computational biology , biochemistry , biology , organic chemistry , enzyme

The genetic incorporation of one azide‐containing and one keto‐containing noncanonical amino acid into a protein at amber and ochre mutation sites respectively, followed by their orthogonal reactions with hydroxylamine‐containing and cyclooctyne‐containing dyes allows highly efficient one‐pot site‐specific dual labeling of the protein in a catalyst‐free fashion.

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