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Competitive Allele‐Specific Hairpin Primer PCR for Extremely High Allele Discrimination in Typing of Single Nucleotide Polymorphisms
Author(s) -
Takei Fumie,
Igarashi Masako,
Oka Yoshimi,
Koga Yusuke,
Nakatani Kazuhiko
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200266
Subject(s) - primer (cosmetics) , typing , biology , allele , genetics , single nucleotide polymorphism , polymerase chain reaction , primer dimer , snp , variants of pcr , microbiology and biotechnology , computational biology , multiplex polymerase chain reaction , genotype , gene , chemistry , organic chemistry
Exploiting the ability of hairpin primer PCR to detect the amount of primer, we have achieved highly allele‐specific PCR by simultaneously using a competitive and a hairpin primer. Moreover, the simple components that are necessary for hairpin primer PCR and operation of this method provide a practical means for rapid SNP typing.