Competitive Allele‐Specific Hairpin Primer PCR for Extremely High Allele Discrimination in Typing of Single Nucleotide Polymorphisms | Zendy

Takei Fumie | Zendy; Igarashi Masako | Zendy; Oka Yoshimi | Zendy; Koga Yusuke | Zendy; Nakatani Kazuhiko | Zendy

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Competitive Allele‐Specific Hairpin Primer PCR for Extremely High Allele Discrimination in Typing of Single Nucleotide Polymorphisms

Author(s) -

Takei Fumie,

Igarashi Masako,

Oka Yoshimi,

Koga Yusuke,

Nakatani Kazuhiko

Publication year - 2012

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201200266

Subject(s) - primer (cosmetics) , typing , biology , allele , genetics , single nucleotide polymorphism , polymerase chain reaction , primer dimer , snp , variants of pcr , microbiology and biotechnology , computational biology , multiplex polymerase chain reaction , genotype , gene , chemistry , organic chemistry

Exploiting the ability of hairpin primer PCR to detect the amount of primer, we have achieved highly allele‐specific PCR by simultaneously using a competitive and a hairpin primer. Moreover, the simple components that are necessary for hairpin primer PCR and operation of this method provide a practical means for rapid SNP typing.

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