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Metabolic Labeling of DNA by Purine Analogues in Vivo
Author(s) -
Neef Anne B.,
Samain Florent,
Luedtke Nathan W.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200253
Subject(s) - dna , deoxyadenosine , in vivo , purine , biochemistry , computational biology , purine metabolism , chemistry , alkyne , biology , genetics , enzyme , catalysis
DNA tracking : The 2′‐deoxyadenosine analogue “EdA” efficiently labels newly synthesized DNA with alkyne groups in vivo without inhibiting cell cycle progression or embryonic development. This provides the first demonstration that a bio‐orthogonal functional group can be metabolically incorporated into DNA through endogenous pathways by addition of a purine analogue to cells.

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