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Universal Caging Group for the in‐Cell Detection of Glutathione Transferase Applied to 19 F NMR and Bioluminogenic Probes
Author(s) -
Ito Mika,
Shibata Aya,
Zhang Jie,
Hiroshima Michio,
Sako Yasushi,
Nakano Yukiko,
KojimaAikawa Kyoko,
Mannervik Bengt,
Shuto Satoshi,
Ito Yoshihiro,
Morgenstern Ralf,
Abe Hiroshi
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200242
Subject(s) - glutathione , bioluminescence , glutathione s transferase , glutathione transferase , chemistry , microanalysis , biochemistry , microbiology and biotechnology , biology , enzyme , organic chemistry
Probing another way: As glutathione transferases (GSTs) are overexpressed in certain tumours and are used as fusion partners, GST detection methods can be used in cancer diagnosis or protein microanalysis. We describe the design of 19 F NMR and bioluminescence probes of GST activity by using the universal caging group. The probes were successfully applied in vitro and in living cells.