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Borononucleotides as Substrates/Binders for Human NMP Kinases: Enzymatic and Spectroscopic Evaluation
Author(s) -
El Amri Chahrazade,
Martin Anthony R.,
Vasseur JeanJacques,
Smietana Michael
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200199
Subject(s) - kinase , context (archaeology) , nucleotide , enzyme , biochemistry , phosphorylation , chemistry , nucleoside , substrate (aquarium) , biology , paleontology , ecology , gene
Borononucleotides are a family of natural nucleotide monophosphate analogues with a 5′‐boronic acid function. As BOP linkages are known to be unstable in solution, we evaluated the ability of borononucleotides to be recognized by nucleoside monophosphate kinases and eventually foil the phosphorylation process. In this context, and with the idea of probing the influence of their size, shape, and flexibility, a library of borononucleotides were synthetized starting from the borononucleotide analogue of thymidine, which was shown to behave as a slow substrate of human TMP kinase. This study thus constitutes a good starting point for the development of new monophosphate mimics as potential substrates or ligands for NMP kinases.