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Nonenzymatic Ligation of DNA with a Reversible Step and a Final Linkage that Can Be Used in PCR
Author(s) -
Engelhart Aaron E.,
Cafferty Brian J.,
Okafor C. Denise,
Chen Michael C.,
Williams Loren Dean,
Lynn David G.,
Hud Nicholas V.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200167
Subject(s) - ligation , dna , chemical ligation , chemistry , polymerase chain reaction , dna polymerase , sequencing by ligation , dna ligase , computational biology , combinatorial chemistry , biochemistry , biology , microbiology and biotechnology , base sequence , gene , genomic library
Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.