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Pan‐Selective Aptamers for the Family of Small GTPases
Author(s) -
Hons Michael,
Niebel Björn,
Karnowski Nora,
Weiche Benjamin,
Famulok Michael
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200164
Subject(s) - aptamer , gtpase , guanine nucleotide exchange factor , nucleotide , conserved sequence , small interfering rna , biology , ras superfamily , rna interference , computational biology , chemistry , rna , microbiology and biotechnology , biochemistry , gene , genetics , base sequence , gtp' , enzyme
Two RNA aptamers , V63 and V88, specifically target the Ras superfamily of small GTPases. Both exhibit pan‐selectivity for different small GTPases, presumably by targeting a conserved sequence motif within the G domain without interfering with nucleotide exchange activity. V63 and V88 are rare examples of pan‐selective aptamers that bind their target with high affinity without affecting its biological activity.