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Development of 5′‐ and 7′‐Substituted Luciferin Analogues as Acid‐Tolerant Substrates of Firefly Luciferase
Author(s) -
Takakura Hideo,
Kojima Ryosuke,
Ozawa Takeaki,
Nagano Tetsuo,
Urano Yasuteru
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200142
Subject(s) - luciferase , bioluminescence , luciferin , luminescence , chemistry , firefly protocol , photoprotein , luminescent measurements , luciferases , biophysics , biochemistry , biology , materials science , optoelectronics , zoology , transfection , gene
Leaving the light on: We have developed acid‐tolerant luciferin analogues with pH‐independent luminescence spectra as well as stable luminescence intensity in response to acidic pH change in the presence of luciferase. These compounds permitted robust bioluminescence microscopic imaging of luciferase‐expressing single cells in an acidic environment typical of those encountered in many biological processes.