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Molecular Drivers of Base Flipping During Sequence‐Specific DNA Methylation
Author(s) -
Matje Douglas M.,
Reich Norbert O.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200104
Subject(s) - dna , methyltransferase , nucleotide , base pair , sequence (biology) , base (topology) , dna methyltransferase , methylation , chemistry , dna methylation , base sequence , sticky and blunt ends , biophysics , computational biology , biology , biochemistry , gene , mathematical analysis , gene expression , mathematics
One step at a time: Substrates containing nucleotide analogues lacking sequence‐specific contacts to the C5 methyltransferase M.HhaI were used to probe the role of individual interactions in effecting conformational transitions during base flipping. A segregation of duties, that is, specific recognition and chemomechanical force for base flipping and active site assembly, within the enzyme is confirmed.