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Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics
Author(s) -
Grammel Markus,
Hang Howard,
Conrad Nicholas K.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200091
Subject(s) - click chemistry , rna , polymerase , nucleoside , chemistry , alkyne , chemical biology , biochemistry , computational biology , biophysics , combinatorial chemistry , biology , dna , gene , catalysis
A versatile “clickable” nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N 6 pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N 6 pA can be visualized and purified by using click chemistry.