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A Universal Expression Tag for Structural and Functional Studies of Proteins
Author(s) -
Rogov Vladimir V.,
Rozenknop Alexis,
Rogova Natalia Yu.,
Löhr Frank,
Tikole Suhas,
Jaravine Victor,
Güntert Peter,
Dikic Ivan,
Dötsch Volker
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200045
Subject(s) - peptide , ubiquitin , protein expression , computational biology , chemistry , cleavage (geology) , microbiology and biotechnology , biochemistry , biology , gene , paleontology , fracture (geology)
Modified ubiquitin sequences , each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide–protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.