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Cytoskeletal Protein Expression and its Association within the Hydrophobic Membrane of Artificial Cell Models
Author(s) -
Martino Chiara,
Horsfall Louise,
Chen Yan,
Chanasakulniyom Mayuree,
Paterson David,
Brunet Adrien,
Rosser Susan,
Yuan YingJin,
Cooper Jonathan M.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201200038
Subject(s) - mreb , cytoskeleton , association (psychology) , expression (computer science) , computational biology , chemistry , actin , microfluidics , aggregate (composite) , cell , nanotechnology , computer science , microbiology and biotechnology , biology , biochemistry , materials science , programming language , philosophy , epistemology
We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.