Cytoskeletal Protein Expression and its Association within the Hydrophobic Membrane of Artificial Cell Models | Zendy

Martino Chiara | Zendy; Horsfall Louise | Zendy; Chen Yan | Zendy; Chanasakulniyom Mayuree | Zendy; Paterson David | Zendy; Brunet Adrien | Zendy; Rosser Susan | Zendy; Yuan YingJin | Zendy; Cooper Jonathan M. | Zendy

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Cytoskeletal Protein Expression and its Association within the Hydrophobic Membrane of Artificial Cell Models

Author(s) -

Martino Chiara,

Horsfall Louise,

Chen Yan,

Chanasakulniyom Mayuree,

Paterson David,

Brunet Adrien,

Rosser Susan,

Yuan YingJin,

Cooper Jonathan M.

Publication year - 2012

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201200038

Subject(s) - mreb , cytoskeleton , association (psychology) , expression (computer science) , computational biology , chemistry , actin , microfluidics , aggregate (composite) , cell , nanotechnology , computer science , microbiology and biotechnology , biology , biochemistry , materials science , programming language , philosophy , epistemology

We demonstrate the cell‐free expression of the actin‐like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water‐in‐oil‐in‐water droplet as happens in vivo. These monodisperse microdroplets were produced on a large scale using microfluidics. We suggest a possible use of this platform for theory validation in fundamental biological studies.

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