Premium
Cover Picture: Bromomaleimide‐Linked Bioconjugates Are Cleavable in Mammalian Cells (ChemBioChem 1/2012)
Author(s) -
Moody Paul,
Smith Mark. E. B.,
Ryan Chris P.,
Chudasama Vijay,
Baker James R.,
Molloy Justin,
Caddick Stephen
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201190090
Subject(s) - rhodamine , bioconjugation , chemistry , förster resonance energy transfer , biomolecule , hela , sortase , nanotechnology , rhodamine b , fluorescence , drug delivery , biophysics , green fluorescent protein , cover (algebra) , fluorescence microscope , combinatorial chemistry , biochemistry , materials science , biology , cell , bacterial protein , mechanical engineering , engineering , physics , quantum mechanics , photocatalysis , gene , catalysis
The cover picture shows a conjugate formed between a dibromomaleimide–rhodamine scaffold and two molecules of green fluorescent protein. Bromomaleimide scaffolds may be used for the synthesis of multifunctionalised biomolecules, and it has been postulated that bromomaleimide adducts are cleaved inside cells. On p. 39 ff., J. R. Baker, J. Molloy, S. Caddick et al. now describe the synthesis of bromomaleimide‐based FRET constructs that upon microinjection into HeLa cells cleave to release free GFP. This process was monitored by quantitative dual‐channel fluorescence video microscopy. Bromomaleimide constructs could therefore be used as cleavable drug delivery systems, or as ratiometric sensors of cytoplasmic delivery. (Cover art designed by Hayley Wood, MRC NIMR, and Dr. Rachel Morgan, UCL).