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KlenTaq DNA Polymerase Adopts Unique Recognition States when Encountering Matched, Mismatched, and Abasic Template Sites: An NMR Study
Author(s) -
Holzberger Bastian,
Pszolla M. Gabriele,
Marx Andreas,
Möller Heiko M.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100802
Subject(s) - dna polymerase , polymerase , dna , dna clamp , computational biology , chemistry , biochemistry , biophysics , biology , polymerase chain reaction , stereochemistry , microbiology and biotechnology , genetics , gene , reverse transcriptase
On the tracks of a DNA polymerase : NMR provides insights into DNA synthesis in a virtually label‐free manner and under close‐to‐physiological conditions. Through the monitoring of the chemical‐shift changes of multiple 13 C‐methyl methionine residues we found unique recognition states for canonical and noncanonical cases, thus indicating enzymatic cycling through distinct paths.