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Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins
Author(s) -
Tae Hyun Seop,
Sundberg Thomas B.,
Neklesa Taavi K.,
Noblin Devin J.,
Gustafson Jeffrey L.,
Roth Anke G.,
Raina Kanak,
Crews Craig M.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100793
Subject(s) - identification (biology) , computational biology , chemistry , degradation (telecommunications) , computer science , biochemistry , combinatorial chemistry , biology , telecommunications , botany
New HyTs are a knockout: We previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.