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Malachite Green Derivatives for Two‐Photon RNA Detection
Author(s) -
Lux Jacques,
Peña Eduardo José,
Bolze Frédéric,
Heinlein Manfred,
Nicoud JeanFrançois
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100747
Subject(s) - malachite green , aptamer , rna , two photon excitation microscopy , chemistry , in vivo , chloride , labelling , combinatorial chemistry , biochemistry , biology , microbiology and biotechnology , organic chemistry , physics , optics , gene , fluorescence , adsorption
The design, preparation and characterisation of a library of malachite green (MG) derivatives for two‐photon RNA labelling is described. Some of these MG derivatives exhibit an increased affinity for an MG‐aptamer, as well as improved two‐photon sensitivity when compared to the classical malachite green chloride. The underlying mechanisms and potential benefits for in vivo RNA visualisation are discussed.