Premium
A General Chemical Ligation Approach Towards Isopeptide‐Linked Ubiquitin and Ubiquitin‐Like Assay Reagents
Author(s) -
Geurink Paul P.,
El Oualid Farid,
Jonker Anika,
Hameed Dharjath S.,
Ovaa Huib
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100706
Subject(s) - ubiquitin , nedd8 , biochemistry , ubiquitins , deubiquitinating enzyme , target protein , neddylation , enzyme , biology , ubiquitin ligase , sumo enzymes , proteases , cullin , native chemical ligation , ubiquitin conjugating enzyme , ubiquitin protein ligases , chemistry , cysteine , gene
Thiolysine‐mediated chemical ligation has generated fluorescence polarisation assay reagents based on isopeptide‐linked ubiquitin‐like protein conjugates. These have been used to monitor the activity of ubiquitin(‐like) proteases. Thus, it is now possible to generate assay reagents that contain substrate‐derived elements around the isopeptide linkage, with no practical limitation.