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Simultaneous Purification and Site‐Specific Modification of Pyrroline‐Carboxy‐Lysine Proteins
Author(s) -
Chiu HsienPo,
Grünewald Jan,
Hao Xueshi,
Brock Ansgar,
Okach Linda,
Uno Tetsuo,
Geierstanger Bernhard H.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100684
Subject(s) - lysine , pyrroline , chemistry , biochemistry , posttranslational modification , combinatorial chemistry , computational biology , stereochemistry , biology , enzyme , amino acid
Sticky residue: Pyrroline‐carboxy‐lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site‐specifically modified with functional probes during the elution process (see scheme).