Premium
Selective Isotopic Unlabeling of Proteins Using Metabolic Precursors: Application to NMR Assignment of Intrinsically Disordered Proteins
Author(s) -
Rasia Rodolfo M.,
Brutscher Bernhard,
Plevin Michael J.
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100678
Subject(s) - isotopic labeling , residue (chemistry) , chemistry , nuclear magnetic resonance spectroscopy , scrambling , nmr spectra database , isotope , computational biology , biochemistry , biology , stereochemistry , computer science , spectral line , organic chemistry , physics , algorithm , quantum mechanics , astronomy
Selective isotopic unlabeling of proteins can provide important residue‐type information as well as reduce congestion of NMR spectra. However, metabolic scrambling often complicates the final isotope‐labeling pattern. Here, an array of metabolic precursors is used to perform robust, residue‐specific unlabeling of proteins. The resulting isotopic‐labeling patterns are predictable and nicely complement NMR experiments that differentiate residue types. This approach has widespread applications, but it is particularly relevant for proteins that lack sequence complexity or a defined tertiary structure.